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Related Experiment Videos

Injectable tissue-engineered cartilage using a fibrin glue polymer.

R P Silverman1, D Passaretti, W Huang

  • 1Division of Plastic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.

Plastic and Reconstructive Surgery
|June 8, 1999
PubMed
Summary
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This study shows fibrin glue can create injectable cartilage. Injecting 40 million chondrocytes per cc of fibrin glue yielded the best quality tissue-engineered cartilage in mice.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Developing injectable scaffolds for cartilage regeneration is crucial.
  • Fibrin glue offers potential as a biocompatible scaffold material.
  • Optimizing cell density within scaffolds is key for tissue formation.

Purpose of the Study:

  • To assess the feasibility of using fibrin glue for injectable cartilage tissue engineering.
  • To determine optimal fibrinogen and chondrocyte concentrations for homogeneous cartilage production.
  • To evaluate the degradation rate of fibrin glue and neocartilage properties.

Main Methods:

  • Fibrin glue was prepared by mixing fibrinogen with thrombin and calcium chloride.
  • Swine chondrocytes were suspended in fibrinogen at varying concentrations (10, 25, 40 million/cc).

Related Experiment Videos

  • Constructs were injected into nude mice, with controls of fibrin glue alone or chondrocytes in saline.

    • Main Results:

    • A fibrinogen concentration of 80 mg/cc showed the most favorable degradation rate.
    • Injecting 40 million chondrocytes/cc resulted in solid, homogeneous neocartilage at 6 and 12 weeks.
    • Neocartilage samples demonstrated presence of type II collagen, with glycosaminoglycan and DNA content comparable to native cartilage.

    Conclusions:

    • Fibrin glue is a feasible and effective polymer for injectable cartilage tissue engineering.
    • A concentration of 40 million chondrocytes/cc is optimal for producing high-quality neocartilage.
    • This injectable approach holds promise for cartilage repair applications.