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Related Experiment Videos

Hemoglobin induction in mouse macrophages.

L Liu1, M Zeng, J S Stamler

  • 1Howard Hughes Medical Institute, Pulmonary and Cardiology Divisions, Duke University Medical Center, Durham, NC 27710, USA.

Proceedings of the National Academy of Sciences of the United States of America
|June 9, 1999
PubMed
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Mammalian cells may have novel globin functions beyond oxygen transport. Studies show beta-minor hemoglobin expression in macrophages, but nitric oxide (NO) consumption did not increase, challenging balanced globin gene expression views.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Hemoglobin's traditional role is oxygen and carbon dioxide transport.
  • Recent research indicates hemoglobin's involvement with nitric oxide (NO).
  • Bacterial flavohemoglobin consumes NO, while mammalian hemoglobin delivers NO.

Purpose of the Study:

  • To investigate alternative functions of globins in mammalian cells.
  • To examine beta-minor hemoglobin subunit expression in murine macrophages.
  • To challenge the established view of balanced alpha- and beta-globin gene expression.

Main Methods:

  • Stimulating murine macrophages with lipopolysaccharide and interferon-gamma.
  • Measuring nitric oxide (NO) production and consumption.

Related Experiment Videos

  • Analyzing beta-minor hemoglobin subunit expression.

    • Main Results:

    • Murine macrophages stimulated with cytokines expressed the beta-minor hemoglobin subunit.
    • Nitric oxide (NO) consumption was not enhanced by cytokine stimulation or hemoglobin expression.
    • Data suggest novel roles for globins in mammalian cellular functions.

    Conclusions:

    • Mammalian globins may possess functions beyond oxygen transport.
    • Beta-minor hemoglobin expression in macrophages does not necessarily correlate with increased NO consumption.
    • The coordinated and balanced expression of alpha- and beta-globin genes is challenged by these findings.