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Updated: Oct 20, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Published on: July 23, 2010
Intranuclear localization of human papillomavirus 16 E7 during transformation and preferential binding of E7 to the
K Smith-McCune1, D Kalman, C Robbins
1Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco CA 94115, USA. kmccune@cc.ucsf.edu
Abstract:
To study intracellular pathways by which the human papillomavirus 16 oncogene E7 participates in carcinogenesis, we expressed an inducible chimera of E7 by fusion to the hormone-binding domain of the estrogen receptor. The chimeric protein (E7ER) transformed rodent fibroblast cell lines and induced DNA synthesis on addition of estradiol. In coimmunoprecipitation experiments, E7ER preferentially bound p130 when compared to p107 and pRb. After estradiol addition, E7ER localization changed to a more intense intranuclear staining. Induction of E7 function was not correlated with binding to p130 or pRb but rather with intranuclear localization and modest induction of binding to p107.
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