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[The relation between mitochondrial DNA mutation and aminogly- coside antibiotics-induced deafness]
1Department of Biology, Nanjing Railway Medical College, Nanjing, 210009 P. R. China. LishanZh@jlonline.com
Abstract
Objective:
To nvestigate the relation between mitochondrial DNA mutation and aminoglycoside antibiotics-induced deafness.
Methods:
Mitochondrial DNA (mtDNA) of peripheral blood from 48 sporadic cases was analysed using PCR-BsmAI digestion and PCR-direct DNA silver sequencing.
Results:
Six of 48 sporadic patients had A to G mutation in 12S rRNA gene of mtDNA at nucleotide 1555.
Conclusion:
The results suggest that mtDNA 1555(G) mutation is related to the occurring of aminoglycoside antibiotics-induced deafness which can cause genetic susceptibility to aminoglycoside antibotics ototoxicity.