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[Screening for mitochondrial 1555(G) mutation in patients with aminoglycoside antibiotic-induced deafness]
1Institute of Otolaryngology,PLA General Hospital, Beijing, 100853 P. R. China. yuanhj@plagh.com.cn
Objective:
To identify the incidence of the 1555(G) mutation in pedigrees and sporadic patients with aminoglycoside antibiotic- induced deafness so as, to privide the theoretical evidence for establishing the method of diagnosis of this disease.
Methods:
Blood samples were obtained from two pedigrees and seven sporadic patients with aminoglycoside antibiotic-induced deafness, and five mothers of the sporadic patients. DNA was extracted from the isolated leukocytes. The mitochondrial DNA fragments were amplified by PCR; 1555(G) mutation was detected by Alw26 I restriction endonuclease digestion.
Results:
Fourteen individuals from two pedigrees carried homoplasmic 1555(G) mutation. Seven sporadic patients and the five mothers did not have 1555(G) mutation.
Conclusion:
The incidence of the 1555(G) mutation in pedigrees with aminoglycoside antibiotic-induced deafness is fairly high, while in sporadic patients is low. Screening for mitochondrial 1555(G) mutation is of potential value to clinical use.