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Related Experiment Video

Updated: May 8, 2026

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

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Published on: April 16, 2019

IL-1 receptor-type expression in relation to atopy.

K Gupta1, A Bewtra

  • 1Creighton University, Department of Medicine/Division of Allergy, Omaha, Nebraska, USA.

The Journal of Allergy and Clinical Immunology
|June 9, 1999
PubMed
Summary
This summary is machine-generated.

Atopic individuals exhibit increased active IL-1 receptors (IL-1RI) and decreased soluble IL-1 receptors, contributing to allergic inflammation. This imbalance in IL-1 receptor forms drives inflammatory responses in allergies.

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Area of Science:

  • Immunology
  • Allergy Research
  • Inflammation Biology

Background:

  • Interleukin-1 (IL-1) mediates inflammation via type I (IL-1RI) and type II (IL-1RII) receptors.
  • Both IL-1 receptors exist in membrane-bound (m) and soluble (s) forms, influencing inflammatory signaling.
  • The balance between active (mIL-1RI) and inactive (mIL-1RII, sIL-1RI, sIL-1RII) receptors is hypothesized to regulate allergic inflammation.

Purpose of the Study:

  • To compare concentrations of membrane-bound IL-1RI and IL-1RII on peripheral blood lymphocytes (PBLs).
  • To quantify soluble IL-1RI, soluble IL-1RII, and IL-1beta in serum and peripheral blood mononuclear cell (PBMC) supernatants.
  • To differentiate these levels between atopic and nonatopic subjects.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was employed.
  • Specific monoclonal antibodies (mAbs) were used for quantification.
  • Measurements included membrane receptors, soluble receptors, and IL-1beta concentrations.

Main Results:

  • Serum IL-1beta levels did not differ between groups.
  • Atopic subjects' PBMCs spontaneously secreted higher IL-1beta levels compared to nonatopic donors.
  • Atopic PBLs showed higher expression of mIL-1RI and mIL-1RII.
  • Nonatopic donors had higher levels of both soluble receptors in serum and PBMCs.

Conclusions:

  • Increased membrane-bound IL-1RI in atopic individuals, coupled with reduced soluble IL-1 receptors, is a key factor in allergic inflammation.
  • This altered receptor profile likely exacerbates inflammatory responses upon allergen exposure.
  • The findings highlight the role of IL-1 receptor balance in the pathogenesis of allergic diseases.