Identification of a 21 kDa laminin-binding component of Candida albicans

Binding of Candida albicans strain JCM0239 to radiolabelled laminin was studied to identify possible receptors. When two forms of the organism were examined, laminin was found to bind to germinating blastoconidia. There was only weak binding to blastoconidia without germ tubes. Laminin-binding to germinating blastoconidia was inhibited by non-labelled laminin or fibrinogen but not by fibronectin, a fibronectin peptide (Gly-Arg-Gly-Asp-Ser), or a laminin peptide (Tyr-Ile-Gly-Ser-Arg). A cell extract from germinating blastoconidia was analyzed by using SDS-PAGE and Western blotting, and 125I-labelled laminin was shown to bind to a 21 kDa component.