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Published on: July 30, 2020
In vitro maturation of baboon oocytes retrieved at the time of cesarean section
R G Brzyski1, M M Leland, C A Eddy
1Department of Obstetrics and Gynecology, The University of Texas Health Science Center at San Antonio, 78284-7836, USA. brzyski@uthscsa.edu
Objective:
To investigate the feasibility of oocyte retrieval at the time of cesarean delivery and the potential of such oocytes to undergo nuclear maturation in vitro using a baboon model and an established culture system.
Design:
Randomized, controlled animal study.
Setting:
Research foundation and university research laboratory.
Animal(S):
Mature pregnant baboons.
Intervention(S):
In vitro culture of aspirated oocytes with or without epidermal growth factor (EGF).
Main Outcome Measure(S):
Oocyte yield, germinal vesicle breakdown, polar body extrusion.
Result(S):
A total of 246 oocytes were retrieved (mean, 35; range, 14-67). Eighty-seven oocytes (35%) underwent germinal vesicle breakdown and 72 oocytes (29%) extruded a polar body. A chi2 analysis revealed no significant effect of EGF on outcome parameters. No effect of gestational age or maternal age on oocyte yield or development was observed.
Conclusion(S):
A sizeable proportion of oocytes obtained from puerperal primates exhibited the capacity to undergo nuclear maturation in vitro.
