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Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli
S Meyer1, N Noisommit-Rizzi, M Reuss
1Institut für Biotechnologie, Universität Halle, Kurt-Mothes-Strasse 3, Halle, D-06120, Germany.
Analytical Biochemistry
|June 11, 1999
Abstract:
To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversed-phase HPLC.