Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A chemogenetic approach to RNA function/structure analysis.

S A Strobel1

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. strobel@csb.yale.edu

Current Opinion in Structural Biology
|June 11, 1999
PubMed
Summary

Nucleotide Analog Interference Mapping uses modified nucleotides to reveal RNA structure and function. This technique identifies RNA helix packing rules, metal ion binding sites, and catalytic mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linguistic analysis of project ownership for undergraduate research experiences.

CBE life sciences education·2012
Same author

Science education. Changing the culture of science education at research universities.

Science (New York, N.Y.)·2011
Same author

Amylin replacement with pramlintide as an adjunct to insulin therapy improves long-term glycaemic and weight control in Type 1 diabetes mellitus: a 1-year, randomized controlled trial.

Diabetic medicine : a journal of the British Diabetic Association·2004
Same author

Exploring the mechanism of the peptidyl transfer reaction by chemical footprinting.

Cold Spring Harbor symposia on quantitative biology·2003
Same author

Biochemical identification of A-minor motifs within RNA tertiary structure by interference analysis.

Biochemical Society transactions·2002
Same author

The human amylin analog, pramlintide, corrects postprandial hyperglucagonemia in patients with type 1 diabetes.

Metabolism: clinical and experimental·2002

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • RNA molecules play crucial roles in various biological processes, including catalysis and gene regulation.
  • Understanding the intricate relationship between RNA sequence, structure, and function is essential for deciphering cellular mechanisms.

Purpose of the Study:

  • To introduce and detail the application of Nucleotide Analog Interference Mapping (NAIM) for elucidating RNA structure-function relationships.
  • To demonstrate the utility of NAIM in uncovering novel aspects of RNA biology.

Main Methods:

  • Synthesis of nearly two dozen nucleotide analogs with alpha-phosphorothioate-tagged triphosphates.
  • Application of the Nucleotide Analog Interference Mapping (NAIM) approach to study RNA molecules.

Related Experiment Videos

Main Results:

  • Identification of new rules governing RNA helix packing.
  • Functional characterization of a binding site for monovalent metal ions within RNA.
  • Elucidation of the catalytic mechanisms employed by RNA enzymes.

Conclusions:

  • Nucleotide Analog Interference Mapping is a powerful method for dissecting RNA structure and function at a chemical level.
  • NAIM provides valuable insights into RNA-protein interactions, metal ion coordination, and enzymatic activity.