Ultrastructural change at rat cerebellothalamic synapses associated with volitional motor adaptation

Our ability to develop or modify motor skills is thought to involve persistent changes in the efficacy of synaptic transmission (synaptic plasticity) in the cerebellum. Previous work from our laboratory and others, examining synapses between neurons in the deep cerebellar nuclei and neurons in the thalamus revealed ultrastructural characteristics that have been implicated in the expression of synaptic plasticity at other locations in the brain. The present study sought evidence of ultrastructural plasticity at cerebellothalamic synapses associated with volitional motor adaptation. Adult rats were subject to 21 days of training, throughout which a novel load (overcome by predominantly shoulder adduction) was applied to the left forelimb while they fed (the right forelimb acted as an internal control). The behavioral paradigm was observed to produce a profound unilateral motor adaptation that was complete by day 15. Three days before the end of training, intracortical microstimulation was performed to identify the regions of primary motor cortex responsible for execution of shoulder adduction movements on the experimental (right) and control (left) sides of the brain. A retrograde neuronal tracer was injected into these cortical regions and the animals were returned to the training cage. Following training, small blocks of thalamic tissue containing retrogradely labeled cells were removed from the brains for ultrastructural analyses of presumed cerebellothalamic synapses (see Materials and Methods section). The only ultrastructural change observed to occur in association with the volitional motor adaptation was an increase in the proportion of dendritic shaft active zone with docked synaptic vesicles.