The development of rabbit diploid cell lines

The authors describe briefly studies on the development of diploid cell lines from fetal tissues (origin: four inbred rabbits). Some lines have been maintained in continuous serial culture for over three years. The Lung 16 may represent a qualifying rabbit diploid cell line when used between cell passages 48 and 100; during this period it has shown a minimum deviation from the normal diploid rabbit karyotype; it supports the growth of rubella virus, is non-tumorigenic and might be useful as a substrate for human vaccine.