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Related Experiment Videos

Tip60 is a nuclear hormone receptor coactivator.

M E Brady1, D M Ozanne, L Gaughan

  • 1Prostate Research Group, Department of Surgery, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom.

The Journal of Biological Chemistry
|June 11, 1999
PubMed
Summary

Tip60 enhances androgen receptor (AR) activity. This protein also coactivates estrogen and progesterone receptors, suggesting Tip60 is a key nuclear hormone receptor coactivator.

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Area of Science:

  • Molecular Biology
  • Endocrinology
  • Cancer Research

Background:

  • The androgen receptor (AR) is crucial for transcriptional control within the nuclear hormone receptor superfamily.
  • Understanding AR transcriptional mechanisms is a key focus in molecular biology.

Purpose of the Study:

  • To identify proteins interacting with the human AR.
  • To investigate the role of Tip60 as a potential coactivator for nuclear hormone receptors.

Main Methods:

  • Yeast two-hybrid screen using the COOH-terminal region of the human AR.
  • Ligand-dependent transactivation assays in LNCaP and COS-1 cell lines.
  • Co-immunoprecipitation assays to confirm protein interactions.

Main Results:

Related Experiment Videos

  • Tip60 was identified as an AR-interacting protein.
  • Tip60 significantly enhances AR-mediated transactivation in a ligand-dependent manner.
  • Tip60 also coactivates the estrogen receptor and progesterone receptor, confirming its role as a nuclear hormone receptor coactivator.
  • Tip60 enhances transactivation comparable to known coactivators like SRC-1, p300, and CBP.

Conclusions:

  • Tip60 functions as a novel coactivator for nuclear hormone receptors, including the AR.
  • Tip60 plays a significant role in enhancing nuclear hormone receptor-mediated transcriptional activation.
  • Further research into Tip60's function may reveal its importance in hormone-related cellular processes.