Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

La autoantigen specifically recognizes a predicted stem-loop in hepatitis B virus RNA.

T Heise1, L G Guidotti, F V Chisari

  • 1Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.

Journal of Virology
|June 11, 1999
PubMed
Summary

Hepatitis B virus (HBV) RNA stability is influenced by the La protein, a nuclear factor. This protein and its fragments bind to specific HBV RNA structures, potentially regulated by inflammatory cytokines.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effects of angiotensin receptor neprilysin inhibition by sacubitril/valsartan on adipose tissue transcriptome and protein expression in obese hypertensive patients.

Scientific reports·2018
Same author

Mixture effects of two plant protection products in liver cell lines.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2018
Same author

Hepatotoxic combination effects of three azole fungicides in a broad dose range.

Archives of toxicology·2017
Same author

Introduction of biosimilar insulins in Europe.

Diabetic medicine : a journal of the British Diabetic Association·2017
Same author

Mixture effects of azole fungicides on the adrenal gland in a broad dose range.

Toxicology·2017
Same author

Improved Insulin Sensitivity With Angiotensin Receptor Neprilysin Inhibition in Individuals With Obesity and Hypertension.

Clinical pharmacology and therapeutics·2016

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Hepatitis B virus (HBV) RNA contains regulatory elements.
  • Nuclear proteins interact with viral RNA.
  • Inflammatory cytokines modulate viral processes.

Purpose of the Study:

  • To identify nuclear proteins binding to HBV RNA.
  • To investigate the role of these proteins in HBV RNA regulation.
  • To determine the interaction mechanism and cytokine influence.

Main Methods:

  • RNA-protein binding assays.
  • Protein purification and sequence analysis.
  • Immunoprecipitation.
  • Competition binding assays.

Main Results:

Related Experiment Videos

  • Three nuclear proteins (p45, p39, p26) identified binding to HBV RNA.
  • p45 is full-length La protein; p39 and p26 are cleavage products.
  • All La protein forms bind to a specific HBV RNA stem-loop structure (nt 1275-1291) in a phosphorylation-dependent manner.
  • Binding affinity (Kd) is approximately 1.0 nM.
  • Protein-RNA interaction and HBV RNA are regulated by gamma interferon and tumor necrosis factor alpha.

Conclusions:

  • The La protein and its fragments bind to a specific site on HBV RNA.
  • La protein binding is phosphorylation-dependent and occurs with high affinity.
  • La protein may enhance HBV RNA stability, both constitutively and in response to inflammatory cytokines.