Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy.

P P Lehenkari1, M A Horton

  • 1Department of Medicine, The Rayne Institute, 5 University Street, University College London, WC1E 6JJ, United Kingdom.

Biochemical and Biophysical Research Communications
|June 12, 1999
PubMed
Summary

Cellular mechanical interactions with the extracellular matrix are crucial. This study measured integrin binding forces on bone cells using atomic force microscopy (AFM), revealing sequence-specific interactions important for cell-matrix communication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A few corrections.

British dental journal·2019
Same author

Electromechanical properties of human osteoarthritic and asymptomatic articular cartilage are sensitive and early detectors of degeneration.

Osteoarthritis and cartilage·2017
Same author

Epizootics of Streptococcus agalactiae infection in captive rays from Queensland, Australia.

Journal of fish diseases·2017
Same author

Interactions between Cooccurring Lactic Acid Bacteria in Honey Bee Hives.

Applied and environmental microbiology·2015
Same author

Nanoscale scraping and dissection of collagen fibrils.

Nanotechnology·2011
Same author

Serological properties of anti-Ly-6.2 serum produced by a new immunization schedule.

Immunogenetics·2011

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Cell-matrix interactions are vital, especially in cells that modify their environment, like bone cells.
  • Osteoclasts rely on integrin receptors for bone degradation, making them a key model for studying these interactions.

Purpose of the Study:

  • To measure integrin binding forces in intact bone cells (osteoclasts and osteoblasts).
  • To investigate the influence of ligand context and environmental factors on these interactions.
  • To demonstrate the utility of Atomic Force Microscopy (AFM) in studying cell-matrix dynamics.

Main Methods:

  • Utilized Atomic Force Microscopy (AFM) to measure integrin binding forces on intact osteoclasts and osteoblasts.
  • Employed various Arg-Gly-Asp (RGD) containing ligands, including peptides and proteins like osteopontin and echistatin.

Related Experiment Videos

  • Varied experimental conditions, including pH and divalent cation composition of the culture medium.

    • Main Results:

    • Measured integrin binding forces ranging from 32 to 97 picoNewtons (pN), demonstrating cell and amino acid sequence specificity.
    • Found binding forces to be saturatable and sensitive to medium pH and divalent cations.
    • Observed that the context of the RGD sequence within larger proteins significantly impacts binding affinity.

    Conclusions:

    • The mechanical properties of cell surface receptor-ligand interactions critically influence cell-matrix cross-talk.
    • AFM is a valuable tool for analyzing cell-matrix interactions in various biological contexts.
    • Findings have implications for understanding bone cell function and receptor-ligand interactions in general.