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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Distribution of human rotavirus G types circulating in Paris, France, during the 1997-1998 epidemic: high prevalence
E Gault1, R Chikhi-Brachet, S Delon
1Laboratoire de Virologie (EA 2391, UFR Saint-Antoine), Hôpital Armand Trousseau, 75571 Paris Cedex 12, France.
Journal of Clinical Microbiology
|June 12, 1999
Insights
Group A rotavirus G genotypes were identified in children with diarrhea. Type G4 was the most common, found in 60% of cases during a Paris study.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Molecular Epidemiology
Background:
- Group A rotavirus is a leading cause of severe diarrheal disease in young children worldwide.
- Genotyping of rotavirus is crucial for understanding transmission dynamics and vaccine efficacy.
Purpose of the Study:
- To determine the G genotypes of Group A human rotavirus in pediatric diarrhea cases.
- To establish the prevalence of different G genotypes in Paris between 1997 and 1998.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used for G genotyping.
- Stool specimens from 170 children with acute diarrhea were analyzed.
Main Results:
- All identified rotavirus isolates belonged to G genotypes G1 to G4.
- Rotavirus G genotype G4 was predominant, accounting for 60% of the cases.
- Genotypes G1, G2, and G3 were also detected, but at lower frequencies.
Conclusions:
- The study identified a G4 predominance in Group A human rotavirus infections among children with diarrhea in Paris.
- These findings contribute to the molecular epidemiology of rotavirus in Europe during the late 1990s.
Abstract:
Group A human rotavirus G genotypes were determined by means of reverse transcription-PCR in 170 stool specimens from children with acute diarrhea admitted to a Paris children's hospital during a 1-year survey (1997 to 1998). The isolates all belonged to types G1 to G4, with type G4 predominating (60%).
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