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A simple HPLC method for simultaneous determination of mycophenolic acid and mycophenolic acid glucuronide in plasma
J O Svensson1, C Brattström, J Säwe
1Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
Therapeutic Drug Monitoring
|June 12, 1999
Abstract:
A reversed-phase high-performance liquid chromatographic method for the simultaneous determination of mycophenolic acid and its metabolite, mycophenolic acid glucuronide, is presented herein. Sample purification is limited to protein precipitation with acetonitrile. The analytes were separated on a C18 column with a mobile phase containing 30% acetonitrile and a 40 mm phosphoric acid buffer at pH 2.1 and measured with UV-detection at 215 nm.