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Small bowel ischaemia-reperfusion increases plasma concentrations of oxidised proteins in rats
F M Abu-Zidan1, C C Winterbourn, M J Bonham
1Department of Surgery, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
Objectives:
To find out whether plasma concentrations of protein carbonyl (a specific marker of oxidative damage of proteins) are increased during intestinal ischaemia-reperfusion and whether they are correlated with von Willebrand's factor (vWF, a marker of endothelial injury) or myeloperoxidase (a marker of neutrophil activation).
Design:
Randomised experimental study.
Setting:
University department of surgery, New Zealand.
Animals:
Thirty anaesthetised adult Wistar rats.
Interventions:
The sham operated group (n = 10) had laparotomy and isolation of the superior mesenteric artery without clamping. The ischaemia-reperfusion group (IR, n = 10) had the superior mesenteric artery clamped for 1 hour and reperfusion for 15 minutes. The control group (n = 10) had direct puncture of the heart to sample blood.
Main Outcome Measures:
Plasma concentrations of protein carbonyl, vWF, and myeloperoxidase.
Results:
Plasma protein carbonyl concentrations were significantly higher in the IR group than in the sham group (p < 0.02, Mann-Whitney test, median (range) 0.187 (0.141-0.242) compared with 0.144 (0.121-0.185) nmol/mg) and in the control group (p < 0.01, Mann-Whitney test, median (range) 0.187 (0.141-0.242) compared with 0.136 (0.108-0.175) nmol/mg). There was a significant correlation between protein carbonyl and vWF concentrations (r = 0.54, F = 10.9, p < 0.003, linear regression) but not with those of myeloperoxidase.
Conclusion:
Intestinal ischaemia-reperfusion caused an increase in the plasma protein carbonyl concentration, which is possibly produced by endothelial cells.