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Related Experiment Videos

Regulation of striatal dopamine receptors by corticosterone: an in vivo and in vitro study.

C H Lammers1, U M D'Souza, Z H Qin

  • 1Genetic Pharmacology Unit, Experimental Therapeutics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 10 Center Drive, MSC 1406, Bethesda, MD 20892-1406, USA.

Brain Research. Molecular Brain Research
|June 15, 1999
PubMed
Summary
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Chronic corticosterone treatment in rats down-regulated D2 dopamine receptors in the dorsal striatum. Glucocorticoids do not directly affect dopamine receptor gene expression.

Area of Science:

  • Neuroscience
  • Endocrinology
  • Molecular Biology

Background:

  • Dopamine receptors are crucial for striatal function.
  • Glucocorticoids, like corticosterone, influence brain function.
  • The direct impact of glucocorticoids on dopamine receptor expression is not fully understood.

Purpose of the Study:

  • To investigate the effects of chronic corticosterone administration and adrenalectomy on dopamine receptor expression in rat striata.
  • To examine the transcriptional regulation of dopamine D1 and D2 receptor genes by glucocorticoids.

Main Methods:

  • In situ hybridization and receptor binding autoradiography were used to assess D1, D2, and D3 receptor expression in rat dorsal and ventral striata.
  • Transient transfection assays in neuroblastoma cell lines were employed to study the transcriptional regulation of D1 and D2 gene promoters by dexamethasone.

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Main Results:

  • Chronic corticosterone administration led to a down-regulation of D2 receptor mRNA in the dorsal striatum after two weeks.
  • No significant changes in D1 or D3 receptor expression were observed in response to corticosterone treatment or adrenalectomy.
  • Dexamethasone showed a minor activation of a small segment of the D2 promoter, but large fragments of D1 and D2 promoters were not regulated.

Conclusions:

  • Glucocorticoids do not appear to exert direct, overall effects on striatal dopamine receptor expression.
  • The observed in vivo down-regulation of D2 receptor mRNA in the dorsal striatum is likely an indirect effect, possibly secondary to increased striatal dopamine release induced by corticosterone.