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[Dynamic changes of nitric oxide and biochemistry in rats with intrahepatic cholestasis]
Objective:
To investigate the correlation between nitric oxide and biochemical changes of intrahepatic cholestasis.
Methods:
Studies were made on endogenous NO, biochemical changes of cholestasis, liver mitochondrial function and renal excretion in a rat model at different time points after ANIT administration.
Results:
In acute reactive phase, serum bilirubin, ALT, AKP, and bile acids significantly increased, accompanied by enhanced plasma NO and renal excretion. Liver NO increased and mitochondrial SDH activity decreased for 2-6 days after ANIT. There was a high negative correlation between liver NO and mitochondrial SDH activity (r = -0.92).
Conclusion:
These results show that higher liver NO may be an important mediator of intrahepatic cholestasis. However, plasma NO increase may play a role in rapidly decreasing serum bilirubin and restoring bile acids, ALT, and AKP to control levels by mediating enhanced renal excretion.