Comparison between a modified ethanol gelation test and protamine sulfate test. Experimental studies.

Area of Science:

• Hematology
• Clinical Chemistry

Background:

• The ethanol gelation test is a common method for detecting fibrin monomers.
• Variations in gel solubility at 37°C have been observed but not fully explained.

Purpose of the Study:

• To compare the diagnostic utility of the protamine sulfate test and a modified ethanol gelation test.
• To elucidate the factors influencing gel solubility in these tests for improved diagnostic accuracy.

Main Methods:

• A comparative experimental study correlating protamine sulfate and modified ethanol gelation tests.
• Observing gel solubility at 37°C after formation at 20°C (Godal and Abildgaard procedure).

Main Results:

• Gel solubility at 37°C varied, indicating different levels of fibrin monomers and fibrinogen.
• The protamine sulfate test demonstrated higher sensitivity than the ethanol gelation test.
• Increased sensitivity of the protamine sulfate test correlated with decreased fibrinogen levels.

Conclusions:

• Gel insolubility reliably indicates the presence of fibrin monomers.
• Gel solubility necessitates the protamine sulfate test to confirm fibrin monomers or high fibrinogen levels.
• These tests provide crucial diagnostic information for coagulation disorders.