Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of changes in lung tissue properties with multiple-indicator dilution.

D L Roerig1, S H Audi, J H Linehan

  • 1Department of Anesthesiology and Pharmacology/Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|June 16, 1999
PubMed
Summary

This study shows that multiple-indicator dilution can detect lung injury by analyzing indicator outflow patterns. These unique patterns help identify specific changes in lung tissue properties caused by inflammation or fluid.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Flurometric Tool to Assess Mitochondrial Redox State of Isolated Perfused Rat Lungs after Exposure to Hyperoxia.

IEEE journal of translational engineering in health and medicine·2014
Same author

An exploration of spatial risk assessment for soil protection: estimating risk and establishing priority areas for soil protection.

The Science of the total environment·2014
Same author

BioInnovate Ireland--fostering entrepreneurial activity through medical device innovation training.

Annals of biomedical engineering·2013
Same author

Is overspecialization a threat?

Wisconsin medical journal·2010
Same author

Lung tissue mitochondrial benzodiazepine receptors increase in a model of pulmonary inflammation.

Lung·2002
Same author

Pulmonary arterial morphometry from microfocal X-ray computed tomography.

American journal of physiology. Heart and circulatory physiology·2001

Area of Science:

  • Pulmonary physiology
  • Pharmacokinetics
  • Medical diagnostics

Background:

  • Lung injury models are crucial for understanding respiratory diseases.
  • The multiple-indicator dilution method offers a way to assess tissue properties non-invasively.
  • Identifying specific tissue changes in lung injury remains a challenge.

Purpose of the Study:

  • To evaluate the utility of multiple-indicator dilution for detecting and identifying lung tissue abnormalities.
  • To determine if different lung injury models produce unique indicator outflow patterns.
  • To explore the potential of specific indicators to reflect changes in lung tissue composition.

Main Methods:

  • Isolated perfused rabbit lungs were used to model lung injury.
  • Four indicators ([14C]diazepam, 3HOH, [14C]phenylethylamine, vascular reference) were injected into the pulmonary artery.

Related Experiment Videos

  • Lung injury was induced by granulomatous inflammation (complete Freund's adjuvant - CFA) or intratracheal fluid instillation.
  • Pulmonary venous outflow concentration-time curves were analyzed using mathematical models.

    • Main Results:

    • Both CFA-induced inflammation and fluid instillation altered lung tissue composition and indicator outflow patterns.
    • Each experimental condition produced a unique pattern in the concentration-time curves.
    • Mathematical modeling yielded distinct parameter vectors for each condition, confirming detectability of changes.
    • A notable finding was a CFA-induced change in diazepam disposition, suggesting increased peripheral-type benzodiazepine receptors.

    Conclusions:

    • The multiple-indicator dilution method, using a carefully selected group of indicators, can effectively detect and identify lung tissue property changes.
    • Unique indicator outflow patterns can differentiate between various lung injury models.
    • This technique holds promise for diagnosing and characterizing lung injuries based on specific tissue alterations.