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Maturation of neuromuscular transmission during early development in zebrafish
P V Nguyen1, L Aniksztejn, S Catarsi
1Centre for Research in Neuroscience, McGill University and Montreal General Hospital Research Institute, Montreal, Quebec H3G 1A4, Canada.
Journal of Neurophysiology
|June 16, 1999
Abstract:
We have examined the rapid development of synaptic transmission at the neuromuscular junction (NMJ) in zebrafish embryos and larvae by patch-clamp recording of spontaneous miniature endplate currents (mEPCs) and single acetylcholine receptor (AChR) channels. Embryonic (24-36 h) mEPCs recorded in vivo were small in amplitude (<50 pA). The rate of mEPCs increased in larvae (3.5-fold increase measured by 6 days), and these mEPCs were mostly of larger amplitude (10-fold on average) with (