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Related Experiment Videos

Multiple regression analysis of interference effects from a hemoglobin-based oxygen carrier solution.

S C Kazmierczak1, P G Catrou, A E Best

  • 1East Carolina University School of Medicine, Department of Pathology and Laboratory Medicine, Greenville, NC 27858, USA. kaz@brody.med.ecu.edu

Clinical Chemistry and Laboratory Medicine
|June 16, 1999
PubMed
Summary

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Clinical chemistry and laboratory medicine·2000

Diaspirin Cross-linked Hemoglobin can interfere with 13 of 29 clinical laboratory tests. This blood substitute requires careful laboratory evaluation to ensure accurate patient results.

Area of Science:

  • Clinical Chemistry
  • Biomedical Engineering
  • Laboratory Medicine

Background:

  • Hemoglobin-based oxygen carriers (HBOCs) are emerging as blood transfusion alternatives.
  • Acellular hemoglobin in HBOCs may cause unique interferences in clinical assays compared to red blood cell lysis.
  • Clinical laboratories need methods to assess HBOC interference for accurate diagnostic testing.

Purpose of the Study:

  • To evaluate the interference potential of Diaspirin Cross-linked Hemoglobin (DCHG) on 29 common clinical laboratory analytes.
  • To identify specific analytes and assay systems affected by DCHG.
  • To establish criteria for clinically significant interference.

Main Methods:

  • DCHG interference was tested on 60 analyte/instrument combinations using Hitachi 747/911, Beckman CX3, Abbott AxSym, Bayer Immuno I, and Dade ACA IV.

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  • Multiple regression analysis classified interferences as analyte-dependent, analyte-independent, or combined.
  • Interference significance was determined using Clinical Laboratory Improvement Amendments of 1988 error criteria.

    • Main Results:

    • Significant interference from DCHG was observed with 13 out of 29 tested analytes.
    • Interference affected albumin, alkaline phosphatase, bilirubin (total and conjugated), cholesterol, total carbon dioxide, iron, lactate dehydrogenase, magnesium, total protein, and triglyceride on Hitachi systems.
    • DCHG also interfered with L-lactate (ACA IV) and glucose (Beckman CX3).
    • Interference plots visually represented DCHG's impact on test results.

    Conclusions:

    • Diaspirin Cross-linked Hemoglobin significantly interferes with numerous clinical laboratory tests.
    • Clinical laboratories must implement interference evaluation protocols for HBOCs to ensure reliable patient test results.
    • Interference plots provide a valuable tool for assessing and reporting HBOC-related assay interferences.