Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peroxynitrite reaction with heme proteins.

M Mehl1, A Daiber, S Herold

  • 1Faculty of Biology, University of Konstanz, Germany.

Nitric Oxide : Biology and Chemistry
|June 16, 1999
PubMed
Summary

Peroxynitrite (PN) inactivates prostacyclin synthase via tyrosine nitration. Iron catalysis, involving a ferryl nitrogen dioxide intermediate, explains this mechanism and suggests P450nor

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of BPaL to treat RR/MDR-TB, pre-XDR-TB, and extensive disease: real-world outcomes from a cohort in Germany.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2026
Same author

Efficacy of BRAF/MEK-inhibitor therapy for epithelioid glioblastoma with a novel BRAFV600 mutation.

Acta neuropathologica communications·2024
Same author

[Air pollution, noise and hypertension : Partners in crime].

Herz·2024
Same author

[Preoperative diagnostics and typing of abdominal soft tissue sarcomas].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2021
Same author

[Influenza].

Der Internist·2019
Same author

Corrigendum to "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162].

Redox biology·2017

Area of Science:

  • Biochemistry
  • Enzymology
  • Oxidative Stress

Background:

  • Peroxynitrite (PN) inactivates heme-thiolate proteins like prostacyclin (PGI2)-synthase through tyrosine nitration.
  • The role of iron catalysis in this inactivation process requires elucidation.

Purpose of the Study:

  • To investigate the involvement of iron catalysis in the interaction of PN with heme-thiolate proteins.
  • To elucidate the reaction mechanism and intermediates formed during PN-protein interactions.

Main Methods:

  • Spectral and kinetic analyses of PN interactions with microperoxidase and P450nor.
  • Competition experiments using phenol to probe reaction pathways.

Main Results:

  • A ferryl nitrogen dioxide complex was identified as a key intermediate.
  • PN decomposition yields dioxygen, nitrite, and nitrate, with P450nor showing higher efficiency.
  • Phenol competed with dioxygen formation, indicating the ferryl intermediate's involvement in both nitration and oxidation pathways.

Conclusions:

  • Iron catalysis, via a ferryl nitrogen dioxide intermediate, mediates PGI2-synthase nitration.
  • P450nor acts as an efficient PN scavenger.
  • Heme-thiolate complexes are favored for trapping PN.

Related Experiment Videos