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Screening oocytes and preimplantation embryos for aneuploidy
1Centre for Preimplantation Genetic Diagnosis, Department of Obstetrics and Gynaecology, Guy's, King's College and St Thomas Hospitals School of Medicine, King's College London, UK.
Current Opinion in Obstetrics & Gynecology
|June 16, 1999
Summary
Maternal age-related aneuploidies decrease in-vitro fertilization success. Preimplantation genetic screening using polar body or embryo biopsy with fluorescence in-situ hybridization can detect common aneuploidies.
Area of Science:
- Reproductive biology
- Genetics
- In-vitro fertilization
Background:
- Fertility declines with increasing maternal age.
- Age-related aneuploidies in female meiosis contribute to reduced pregnancy and live birth rates.
- Aneuploidies are chromosomal abnormalities that can arise during egg cell division.
Purpose of the Study:
- To investigate the impact of maternal age on aneuploidies.
- To explore methods for screening aneuploidies in oocytes and preimplantation embryos.
- To assess the feasibility of improving in-vitro fertilization outcomes through genetic screening.
Main Methods:
- Polar body biopsy and multicolour fluorescence in-situ hybridization (FISH) for oocyte screening.
- Cleavage stage biopsy and multicolour FISH for preimplantation embryo screening.
- Analysis of common aneuploidies in relation to maternal age.
Main Results:
- Aneuploidy rates increase with advancing maternal age.
- Polar body and cleavage stage biopsy with FISH are viable methods for detecting aneuploidies.
- Screening can identify embryos with chromosomal abnormalities.
Conclusions:
- Maternal age-related aneuploidies are a significant factor in declining in-vitro fertilization success.
- Preimplantation genetic screening offers a potential strategy to improve IVF outcomes.
- Technological advancements in biopsy and FISH enable the detection of aneuploidies in reproductive cells and early embryos.