Enhancement of Mycoplasma bovis detection in milk samples by antigen capture prior to PCR
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Updated: Aug 23, 2026
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
1Division 4, Federal Institute for Health Protection of Consumers and Veterinary Medicine, Naumburger Str. 96a, D-07743 Jena, Germany.
Abstract:
A rapid and sensitive procedure to detect Mycoplasma bovis from milk samples was developed. Mycoplasma cells were collected by selective binding to a monoclonal antibody, thus separating them from milk proteins and facilitating DNA extraction. Polymerase chain reaction (PCR) using species-specific primers allowed detection of 20 cfu ml-1 in agarose gels and 2 cfu ml-1 on Southern blots. The method is especially suitable for specimens from subclinical cases and intermittent shedders.
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