Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The zebrafish eya1 gene and its expression pattern during embryogenesis.

I Sahly1, P Andermann, C Petit

  • 1Unité de Génétique des Déficits Sensoriels, CNRS URA 1968, Institute Pasteur, 25 rue du Dr. Roux, F-75724 Paris Cedex 15, France. cpetit@pasteur.fr

Development Genes and Evolution
|June 17, 1999
PubMed
Summary

The zebrafish eya1 gene, similar to human EYA1, is crucial for development. Its conserved expression pattern in sensory organs and branchial arches suggests a vital, evolutionarily preserved function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azacitidine-venetoclax versus azacitidine salvage treatment for primary induction failure or first relapsed acute myeloid leukaemia patients.

European journal of haematology·2023
Same author

Radiation therapy in the thoracic region: Radio-induced cardiovascular disease, cardiac delineation and sparing, cardiac dose constraints, and cardiac implantable electronic devices.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2023
Same author

Reply to letter by Hadigal et al. regarding the cost-effectiveness of high dose quadrivalent vaccine in three European countries.

Journal of medical economics·2023
Same author

Interparticle Forces of a Native and Encapsulated Metal-Organic Framework and Their Effects on Colloidal Dispersion.

ACS applied materials & interfaces·2021
Same author

Extended embryo culture is effective for patients of an advanced maternal age.

Scientific reports·2021
Same author

Zinc and cadmium accumulation in controlled crosses between metallicolous and nonmetallicolous populations of Thlaspi caerulescens (Brassicaceae).

The New phytologist·2021

Area of Science:

  • Developmental Biology
  • Genetics
  • Comparative Genomics

Background:

  • Eyes absent-like (EYA) genes encode transcriptional coactivators.
  • Human EYA1 haploinsufficiency causes branchio-oto-renal syndrome.
  • Understanding EYA gene function in lower vertebrates is crucial.

Purpose of the Study:

  • To isolate and characterize the zebrafish homologue of the EYA1 gene (eya1).
  • To investigate the expression pattern of zebrafish eya1 during embryogenesis.

Main Methods:

  • Isolation of zebrafish eya1 cDNA.
  • Analysis of eya1 gene expression via whole-mount in situ hybridization during zebrafish embryogenesis.

Main Results:

  • Zebrafish eya1 shares 84.7% protein identity with human EYA1.

Related Experiment Videos

  • eya1 transcripts are detected in cranial placodes (olfactory, otic, lateral line), anterior pituitary, somites, pectoral fins, and branchial arches.
  • Expression in the developing ear is localized to the otic vesicle, and in the lateral line, it is found in placodes, ganglia, and differentiating cells.

    • Conclusions:

    • Zebrafish eya1 exhibits a highly conserved expression pattern compared to its mammalian counterparts.
    • This conservation suggests that the function of the EYA1 gene has been maintained throughout vertebrate evolution.
    • The study provides a foundation for further functional analysis of eya1 in zebrafish.