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Protein encapsulation in biodegradable amphiphilic microspheres
P Bouillot1, N Ubrich, F Sommer
1Laboratoire de Chimie-Physique Macromoléculaire, UMR CNRS-INPL 7568, groupe ENSIC, BP 451, 54 001, Nancy cedex, France. p.bouillot@bris.ac.uk
Abstract:
MPOE-PLA microspheres containing bovine serum albumin (BSA) were prepared by the double emulsion method with high encapsulation efficiency ( approximately 93%). Confocal scanning microscopic analysis using MPOE-PLA labelled with 1-pyrenemethanol showed the MPOE coating of the microsphere surface. This coating improves the performance of the release system compared with PLA microspheres; the hydrophilic chains reduce the BSA adsorption onto the microspheres and increase the amount of BSA released in the supernatant. Microsphere analysis using atomic force microscopy showed that the presence of the MPOE chains also leads to surface roughness. Studies of the diffusion of 1% rhodamine aqueous solution into the microspheres by means of confocal microscopy showed a fast diffusion of water through the matrices containing high molecular weight MPOE chains (?10 000 g mol-1) and could explain the fast release of BSA from these microspheres.
Insights
Methoxy Poly(ethylene oxide)-Poly(lactic acid) (MPOE-PLA) microspheres enhance bovine serum albumin (BSA) release. The MPOE coating improves drug delivery performance by reducing adsorption and increasing BSA release, offering a promising drug delivery system.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Polymer Chemistry
Background:
- Poly(lactic acid) (PLA) microspheres are widely used for drug delivery.
- Controlling drug release kinetics and minimizing non-specific adsorption remain challenges.
- Methoxy Poly(ethylene oxide)-Poly(lactic acid) (MPOE-PLA) copolymers offer tunable properties for improved performance.
Purpose of the Study:
- To prepare and characterize MPOE-PLA microspheres for enhanced bovine serum albumin (BSA) delivery.
- To investigate the effect of MPOE coating on microsphere surface properties and BSA release kinetics.
- To elucidate the relationship between MPOE chain characteristics and drug diffusion within the microspheres.
Main Methods:
- Double emulsion method for MPOE-PLA microsphere preparation.
- Confocal scanning microscopy for surface analysis and MPOE coating visualization.
- Atomic force microscopy (AFM) for surface roughness assessment.
- Diffusion studies using rhodamine aqueous solution to evaluate water penetration.
Main Results:
- High encapsulation efficiency (~93%) achieved for BSA-loaded MPOE-PLA microspheres.
- MPOE coating confirmed on microsphere surfaces, leading to reduced BSA adsorption and increased BSA release.
- Surface roughness was observed due to the presence of MPOE chains.
- Fast water diffusion through MPOE-PLA matrices, particularly with high molecular weight MPOE, correlated with rapid BSA release.
Conclusions:
- MPOE-PLA microspheres demonstrate improved performance as a drug delivery system compared to plain PLA.
- The MPOE hydrophilic chains effectively reduce protein adsorption and enhance drug release.
- The findings suggest MPOE-PLA microspheres are a promising platform for controlled protein delivery.