Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

3'-End processing of pre-mRNA in eukaryotes.

E Wahle1, U Rüegsegger

  • 1Institut für Biochemie, Martin-Luther-Universität Halle-Wittenberg, Germany. ewahle@biochemtech.uni-halle.de

FEMS Microbiology Reviews
|June 17, 1999
PubMed
Summary

Eukaryotic messenger RNA (mRNA) 3' end processing involves cleavage and polyadenylation, crucial for gene expression. Key sequence elements and protein factors are involved, though the endonuclease remains unidentified.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Block of HAC1 mRNA translation by long-range base pairing is released by cytoplasmic splicing upon induction of the unfolded protein response.

Cell·2001
Same author

Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.

The EMBO journal·2000
Same author

Nuclear inclusions in oculopharyngeal muscular dystrophy consist of poly(A) binding protein 2 aggregates which sequester poly(A) RNA.

Human molecular genetics·2000
Same author

The Saccharomyces cerevisiae RNA-binding protein Rbp29 functions in cytoplasmic mRNA metabolism.

The Journal of biological chemistry·2000
Same author

The nuclear poly(A) binding protein, PABP2, forms an oligomeric particle covering the length of the poly(A) tail.

Journal of molecular biology·2000
Same author

Cap-dependent deadenylation of mRNA.

The EMBO journal·2000

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Eukaryotic mRNA 3' end formation requires endonucleolytic cleavage and polyadenylation.
  • This process is directed by specific sequence elements like AAUAAA and GU-rich regions.
  • Several protein factors and their interactions are known, but the endonuclease is unidentified.

Purpose of the Study:

  • To elucidate the molecular mechanisms of mRNA 3' end processing.
  • To identify the endonuclease involved in cleavage and polyadenylation.
  • To analyze the cooperative interactions within the processing complex.

Main Methods:

  • Purification of processing factors.
  • Cloning of cDNAs for subunits.
  • Analysis of RNA-protein and protein-protein interactions.
  • Investigating regulated polyadenylation.
  • Comparing mammalian and yeast systems.

Main Results:

  • Six factors identified for cleavage and polyadenylation; 11 subunits cloned.
  • Cooperative interactions within the processing complex partially analyzed.
  • Regulated polyadenylation examples are under molecular analysis.
  • Yeast processing sequences are more degenerate; downstream elements absent.

Conclusions:

  • mRNA 3' end processing is complex, involving multiple factors and sequence elements.
  • The endonuclease identity and full functional mechanisms require further investigation.
  • Processing is coupled to transcription via interactions with mRNA cap and RNA polymerase II.

Related Experiment Videos