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Related Experiment Videos

Heparin and low molecular weight heparin decrease nitric oxide production by human polymorphonuclear cells.

A E Beltrán1, F Concepción, D Manzanares

  • 1Laboratorio de Farmacología, Centro de Química Farmacéutica, La Habana, Cuba. cqf@infomed.sld.cu

Archives of Medical Research
|June 18, 1999
PubMed
Summary

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Heparin and its derivatives reduce nitric oxide (NO) production in human immune cells. This suggests potential therapeutic benefits for inflammatory diseases involving NO.

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Heparin and low molecular weight heparin (LMWH) derivatives possess biological functions beyond anticoagulation.
  • These functions include modulating leukocyte adhesion, activation, and trafficking.

Purpose of the Study:

  • To investigate the in vitro effects of heparin and LMWH on nitric oxide (NO) production.
  • Focus on human polymorphonuclear leukocytes (PMN) and their response to stimulation.

Main Methods:

  • Stimulation of human PMN using N-formyl-methionyl-leucyl-phenylalanine (fMLP).
  • Measurement of NO production in response to varying concentrations of heparin and LMWH.
  • Assessment of nitric oxide synthase (NOS) activity.

Main Results:

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  • Heparin significantly decreased fMLP-stimulated NO production at 0.5 and 5 µg/mL.
  • LMWH demonstrated inhibitory effects only at higher concentrations (50 and 200 µg/mL).
  • The mechanism of inhibition by LMWH was independent of NOS activity.

    • Conclusions:

    • Heparin and LMWH derivatives can modulate NO production in PMN.
    • These findings support the potential therapeutic use of heparin and LMWH in inflammatory conditions where NO is a key mediator.