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Updated: Aug 17, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Cell-density (cycle) dependent silencer of the rat insulin-like growth factor binding protein-2 (IGFBP-2) gene
E Kutoh1, J Margot, J Schwander
1Molekulare Endokrinologie, Zentrum für Lehre und Forschung, Kantonsspital Basel, Switzerland. ekuto@janbe.jnj.com
Abstract:
An element which has a negative effect on transcription has been identified in the 5'-flanking region of the rat insulin-like growth factor binding protein-2 (IGFBP-2) gene. This element was confirmed to be a silencer by truncation or by linking it to a heterologous promoter. The silencer activity disappeared in the growth-arrested BRL-3A cells at the G1/S border by high cell density where the IGFBP-2 production is highly elevated. This observation may represent a novel mechanism through which gene expression is controlled by modulation of a silencer. Taken together, these results suggest a regulatory link between cell growth and IGFBP-2 expression regulated by its silencer.
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