Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Intravitreally injected platelet activating factor induces retinitis in experimental animals.

J T Rosenbaum¹, E Angell, D Wilson

¹Department of Medicine, Oregon Health Sciences University, Casey Eye Institute, Portland 97201-4197, USA. rosenbaj@ohsu.edu

Current Eye Research

|June 18, 1999

Summary

Platelet activating factor (PAF) induces significant intraocular inflammation, particularly in the retina and choroid, in laboratory animals. These findings suggest PAF is a key mediator in retinal inflammation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cytotoxicity of new aza-alkyl lysophospholipids against drug-sensitive and resistant human ovarian tumor-cell lines - role of free-radicals and potentiation of cytotoxicity by tnf-alpha and cddp.

Oncology reports·2011

Same author

Membrane cholesterol content and sensitivity of human carcinoma-cells to antineoplastic ether phospholipids.

International journal of oncology·2011

Same author

Cytotoxic activity against resistant human tumor-cell lines by 2 synthetic demethylpodophyllotoxin derivatives - a single stereoisomeric change prevents cell-death by apoptosis.

International journal of oncology·2011

Same author

Effect of paf antagonists on the cytotoxic activity of antineoplastic ether phospholipids.

International journal of oncology·2011

Same author

New antineoplastic aza-phospholipids - synthesis, in-vitro cytotoxicity and differential cellular-sensitivity against 70 human tumor-cell lines.

International journal of oncology·2011

Same author

Sensitivity of drug-resistant B-cell lines from AIDS-related non-hodgkins-lymphoma to newly synthesized podophyllotoxin derivatives and aza-alkyllysophospholipids - enhanced sensitization by pretreatment with interferon-gamma.

International journal of oncology·2011

Area of Science:

Ophthalmology
Immunology
Lipid Mediators

Background:

Platelet-activating factor (PAF) is a lipid mediator implicated in anterior uveitis.
The precise role of PAF in intraocular inflammation requires further investigation.

Purpose of the Study:

To characterize the histological changes resulting from intravitreal injection of PAF, its analogs, or lyso-PAF in rabbits and rats.
To elucidate the role of PAF in intraocular inflammation, specifically focusing on retinal and choroidal involvement.

Main Methods:

Intravitreal injections of ethoxy PAF (a stable PAF analog), PAF, lyso-PAF, and control lipids were administered to laboratory rats and rabbits.
Doses ranged from 1 ug to 5 mg, with observations at time points from 6 hours to 7 days post-injection.
Histological examination was performed to assess the inflammatory response.

Related Experiment Videos

Main Results:

Ethoxy PAF (100 ug) consistently induced marked uveitis, with inflammation predominantly in the retina and choroid in both species.
Intravitreal PAF (1 mg) or lyso-PAF (1 mg) also induced retinitis in rabbits.
An inactive lipid control did not induce retinal inflammation, distinguishing PAF-induced changes from those caused by IL-1 or endotoxin.

Conclusions:

PAF appears to be a significant mediator of retinal inflammation.
PAF antagonists have shown efficacy in inhibiting retinal toxicities, supporting PAF's role.
Further research into PAF's role in intraocular inflammatory diseases is warranted.