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Related Experiment Videos

Long-lasting decrease of marrow and circulating long-term culture initiating cells after allogeneic bone marrow

C Selleri1, J P Maciejewski, G De Rosa

  • 1Division of Hematology, Federico II University School of Medicine, Naples, Italy.

Bone Marrow Transplantation
|June 18, 1999
PubMed
Summary
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Hematopoietic stem cell compartments remain significantly reduced long-term after bone marrow transplant. Circulating stem cell counts can reflect bone marrow stem cell status post-transplant.

Area of Science:

  • Hematology
  • Transplantation Immunology
  • Stem Cell Biology

Background:

  • Allogeneic bone marrow transplant (BMT) aims for full hematopoietic reconstitution.
  • Long-term effects on stem cell populations post-BMT require further investigation.

Purpose of the Study:

  • To quantify and compare hematopoietic progenitor cells in bone marrow (BM) and peripheral blood (PB) of BMT patients versus healthy donors.
  • To assess the long-term impact of BMT on stem cell compartments using colony-forming unit-granulocyte-macrophage (CFU-GM) and long-term culture initiating cell (LTC-IC) assays.

Main Methods:

  • Flow cytometry to analyze CD34+ cells.
  • Methylcellulose colony assay for CFU-GM.
  • Long-term culture initiating cell (LTC-IC) assay for early progenitor cells.

Related Experiment Videos

  • Study included 37 normal donors and 25 patients 1-8 years post-BMT.

    • Main Results:

    • BM CD34+ cells were 3-4 fold decreased; PB CD34+ cells were unchanged in BMT patients.
    • BM CFU-GM were 2.1 fold decreased; PB CFU-GM showed marginal decrease.
    • LTC-IC assay revealed an 8-fold decrease in BM and 5-fold decrease in PB progenitor cells in BMT patients.
    • BM LTC-IC correlated with BM CD34+, BM CFU-GM, and PB LTC-IC.

    Conclusions:

    • Early stem cell compartments (LTC-IC) are persistently depressed long-term after successful BMT.
    • Enumerating circulating LTC-IC may serve as a reliable indicator of the bone marrow stem cell compartment post-transplant.
    • Hematopoietic reconstitution can be sustained by a reduced stem cell pool following BMT.