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Related Experiment Videos

Zebrafish genetic map with 2000 microsatellite markers.

N Shimoda1, E W Knapik, J Ziniti

  • 1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.

Genomics
|June 22, 1999
PubMed
Summary
This summary is machine-generated.

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Researchers developed over 2000 microsatellite markers for zebrafish, enabling high-resolution genetic mapping. This advances the positional cloning of genes critical for vertebrate embryonic development.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Zebrafish are a key vertebrate model for large-scale genetic screens.
  • Over 1800 recessive mutations affecting embryonic development have been identified.
  • Efficient cloning of these mutant genes requires a dense genetic map.

Purpose of the Study:

  • To develop a high-density genetic map for zebrafish.
  • To facilitate the positional cloning of developmental genes.

Main Methods:

  • Development and application of over 2000 microsatellite (CA) repeat markers.
  • Genetic mapping to achieve an average resolution of 1.2 centimorgans (cM).

Main Results:

  • A genetic map with 2000 markers providing 1.2-cM average resolution was established.

Related Experiment Videos

  • This resolution is sufficient for initiating positional cloning of many zebrafish mutations.
  • One centimorgan in zebrafish corresponds to approximately 0.74 megabases.

    • Conclusions:

    • The developed genetic map significantly enhances the ability to clone genes identified in zebrafish screens.
    • This resource accelerates research into vertebrate embryonic development and gene function.