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Detection of Fas/APO-1 in six human urogenital malignant cell lines with flow cytometry
1Department of Urology, First Hospital of Beijing Medical University, Beijing Medical University, China.
Objective:
To investigate the expression of Fas/APO-1 in urogenital tumor cell lines.
Methods:
With direct immunofluorescence, the expression of Fas/APO-1 in six urogenital malignant cell lines and one primary in vitro cultured normal renal fibroblast was detected by flow cytometry.
Results:
Expression of Fas/APO-1 was detected in all six urogenital tumor cell lines, but with limited positive cell percentage and relatively lower fluorescence intensity, compared with expression of Fas/APO-1 in normal control of primary in vitro cultured renal fibroblast.
Conclusions:
Lower expression of Fas/APO-1 in urogenital malignant cell lines than that in normal cells might be the reason for occurrence and progression of urogenital malignant tumors.
