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Updated: Aug 9, 2026
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Immunohistochemical studies on the endotoxin-induced uveitis
P Yang1, A F de Vos, A Kijlstra
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Laboratory of Health Ministry of P. R. China, Guangzhou, China.
Objective:
To investigate the longitudinal changes of macrophages and major histocompatibility complex (MHC) class II-positive cells in the iris and ciliary body of Lewis rats after lipopolysaccharide (LPS) injection.
Methods:
Immunohistochemistry was performed using monoclonal antibodies to monocytes and macrophages (ED1) and MHC class II-positive cells (OX6) on wholemounts of the iris and ciliary body in endotoxin-induced uveitis (EIU).
Results:
A network of macrophages (ED1+ cell) and MHC class II-positive cells, was present in the iris and ciliary body of normal Lewis rats. Most cells in the iris and ciliary body displayed dendritiform appearance. A severe involvement of the iris and ciliary body, as evidenced by a rapid influx of monocytes and macrophages and remarkable increase of MHC class II-positive cells, was observed after LPS injection.
Conclusions:
A network of macrophages and MHC class II-positive cells in the iris and ciliary body may play an important role in immune surveillance. LPS injection induces a severe inflammation in the anterior segment of the eye, which may serve as a model for acute anterior uveitis in human.
