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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Immunohistochemical studies on the endotoxin-induced uveitis
P Yang1, A F de Vos, A Kijlstra
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Laboratory of Health Ministry of P. R. China, Guangzhou, China.
Chinese Medical Journal
|June 22, 1999
Summary
Macrophages and MHC class II-positive cells form a network in the rat eye, crucial for immune surveillance. Lipopolysaccharide (LPS) injection causes severe anterior segment inflammation, modeling human acute anterior uveitis.
Area of Science:
- Ophthalmology
- Immunology
- Cell Biology
Background:
- Macrophages and MHC class II-positive cells are present in the iris and ciliary body of normal Lewis rats.
- These cells often exhibit a dendritiform morphology.
Purpose of the Study:
- To examine longitudinal changes in macrophages and MHC class II-positive cells in the iris and ciliary body.
- To investigate these changes following lipopolysaccharide (LPS) administration.
Main Methods:
- Immunohistochemistry was employed using ED1 and OX6 monoclonal antibodies.
- The study focused on wholemounts of the iris and ciliary body in endotoxin-induced uveitis (EIU) models.
Main Results:
- A network of macrophages (ED1+) and MHC class II-positive cells (OX6+) was identified in normal rat irises and ciliary bodies.
- LPS injection led to a significant influx of monocytes/macrophages and increased MHC class II-positive cells.
- Severe inflammation of the iris and ciliary body was observed post-LPS.
Conclusions:
- The iris and ciliary body macrophage/MHC class II network likely plays a role in ocular immune surveillance.
- LPS-induced anterior segment inflammation serves as a valuable model for human acute anterior uveitis.
