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[Experimental study on prevention of pneumoconiosis complicating with tuberculosis by Mycobacterium vaccae]
Objective:
To explore the role of Mycobacterium vaccae in prevention of pneumoconiosis complicating with tuberculosis.
Method:
Mycobacterium vaccae was injected into rats which had been exposed to quartz for 2 weeks, and H37Rv was injected into their tail veins 1 month later. All the rats were killed 3 months later. Indexes for evaluation in the study included tuberculous lesion pathological change index, Mycobacterium tuberculosis culture in a fixed amount in lung tissues, count of Mycobacterium tuberculosis and histopathological changes in alveolar macrophages.
Result:
Tuberculous lesion pathological change index, Mycobacterium tuberculosis culture in a fixed amount in lung tissues and count of Mycobacterium tuberculosis in alveolar macrophages were 2.6 +/- 0.5, (4.40 +/- 4.00) x 10(4) CFU and 7.2 +/- 3.2 respectively in pneumoconiosis complicated with tuberculosis and injecting Mycobacterium vaccae group, while 3.1 +/- 0.3, (18.9 +/- 18.2) x 10(4) CFU and 12.5 +/- 6.3 respectively in the control group. And statistically significant differences were found between the two groups (P < 0.05, 0.01, 0.05 respectively). The histopathological analysis revealed that proliferative and lymphoid nodules were predominant in the pneumoconiosis complicated with tuberculosis and injecting Mycobacterium vaccae group, while necrotic nodules in the control group.
Conclusion:
Mycobacterium vaccae might play a role in prevention of pneumoconiosis complicating with tuberculosis.