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Updated: Aug 5, 2026

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
Published on: July 3, 2013
Endothelium-dependent effect of bradykinin and ATP on rabbit ventricular myocytes
Y J Ou1, Q X Shan, J P Bourreau
1Department of Physiology and Institute of Cardiovascular Sciences & Medicine, The University of Hong Kong, SAR of China.
Abstract:
We have investigated the effects of bradykinin (BK) and ATP on Ca2+ transient induced by electrical-field stimulation in freshly isolated rabbit ventricular myocytes, in the presence or absence of rabbit aortic endothelial cells. BK and ATP induced an increase in intracellular Ca2+ concentration ([Ca2+]i) in the endothelial cells, but had no significant effect on Ca2+ transient in electrical-field stimulated ventricular myocytes. In the presence of cultured endothelial cells, the amplitude of Ca2+ transient induced by electrical stimulation in ventricular myocytes was decreased. BK and ATP further reduced the amplitude of Ca2+ transient induced by electrical stimulation in ventricular myocytes. These data show that BK and ATP have endothelium-dependent depressing effects on ventricular myocytes and indicate that substances released from endothelial cells in response to BK and ATP stimulation can modulate ventricular myocytes excitation-contraction coupling. However, identification of the cardioactive mediators produced by the endothelial cells requires further study.
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