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Updated: Aug 16, 2026
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Studies on the new cell line TT-I: viral susceptibility and chromosomal changes related to mycoplasma contamination
Abstract:
For understanding some biological properties of TT-1 cell line established in our laboratory in 1962, its viral susceptibility and the changes in chromosomes related to mycoplasma contamination were studied. TT-1 cells were susceptible to the infection of picorna (Polio type 1, Coxsackie B1 and Echo type 2), measles, Newcastle disease, herpes simplex (type 1), adeno (type 12) and vaccinia viruses. Contamination of TT-1 cells with Mycoplasma hominis was treated successfully with bottromycin A2 (50 microgram/ml). The chromosomes of the cells ranged from 52 to 55 in number and two additional minute chromosomes were found in the cells contaminated with the mycoplasma. However, after treatment with bottromycin, these two minute chromosomes disappeared.
Insights
This study investigated the TT-1 cell line
Area of Science:
- Cell biology
- Virology
- Cytogenetics
Background:
- The TT-1 cell line, established in 1962, requires characterization of its biological properties.
- Understanding viral susceptibility and chromosomal changes is crucial for cell line integrity.
- Mycoplasma contamination can significantly alter cellular characteristics.
Purpose of the Study:
- To determine the viral susceptibility of the TT-1 cell line.
- To investigate chromosomal alterations associated with Mycoplasma contamination in TT-1 cells.
- To assess the efficacy of bottromycin A2 in treating Mycoplasma contamination and reversing chromosomal changes.
Main Methods:
- Viral susceptibility testing using various virus types (Picornaviruses, measles, Newcastle disease, herpes simplex, adenovirus, vaccinia).
- Cytogenetic analysis of TT-1 cells before and after Mycoplasma contamination.
- Treatment of Mycoplasma hominis contamination with bottromycin A2 (50 µg/ml).
- Post-treatment chromosomal analysis to observe recovery.
Main Results:
- TT-1 cells demonstrated susceptibility to a broad range of viruses, including picorna, measles, Newcastle disease, herpes simplex, adeno, and vaccinia viruses.
- Mycoplasma hominis contamination was identified in TT-1 cells.
- Contaminated cells exhibited abnormal chromosome numbers (52-55) with two additional minute chromosomes.
- Treatment with bottromycin A2 successfully eradicated Mycoplasma hominis and resulted in the disappearance of the two minute chromosomes.
Conclusions:
- The TT-1 cell line is broadly susceptible to various viruses.
- Mycoplasma contamination induces specific chromosomal abnormalities in TT-1 cells.
- Bottromycin A2 is an effective treatment for Mycoplasma contamination, restoring normal chromosomal structure in TT-1 cells.
