Dual effects of pentobarbital on rat sacral dorsal commissural neurons in vitro

Z P Pang¹, T L Xu, G Y Hu

¹Department of Anatomy, K K Leung, Brain Research Centre, Fourth Military Medical University, Xi-an, China.

Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

|June 22, 1999

Abstract

Aim:

To study the effects of pentobarbital (PB) on acutely dissociated rat sacral dorsal commissural neurons (SDCN).

Methods:

Nystatin-perforated patch clamp recording was used.

Results:

(1) At a holding potential of -40 mV, PB induced inward Cl- current (IPB) in a concentration-dependent manner with a EC50 (95% confidence limits) of 416 (385-477) mumol.L-1 and a Hill coefficient of 1.08. (2) Picrotoxin reversibly blocked IPB. (3) The reversal potential of IPB was close to the Cl- equilibrium potential. (4) PB enhanced GABA-induced Cl- influx (IGABA). In the presence of PB 30 mumol.L-1, the EC50 (95% confidence limits) of IGABA decreased from 6.9 (5.4-8.4) mumol.L-1 to 3.5 (2.9-4.1) mumol.L-1.

Conclusion:

PB had dual effects on SDCN, facilitated GABAA receptor-mediated currents and at higher concentrations induced Cl- influx itself.

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Dual effects of pentobarbital on rat sacral dorsal commissural neurons in vitro

Aim:

Methods:

Results:

Conclusion:

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