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Effect of mitoxantrone on DNA polymerase of Ehrlich ascites carcinoma cells
1Cancer Institute, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
Aim:
To study the effect of mitoxantrone (Mit) on DNA polymerases of tumor cells.
Methods:
DNA polymerases of Ehrlich ascites carcinoma cells were isolated by phosphocellulose column chromatography. The effects of Mit on DNA polymerase alpha, beta, and gamma were detected by method of K Ono.
Results:
Mit inhibited DNA polymerase alpha, beta, and gamma, IC50 values were 11.9, 6.5, and 11.9 mumol.L-1, and Ki 1.86, 2.22, and 2.05 mumol.L-1, respectively. The inhibitory mode of Mit on DNA polymerase alpha, beta, and gamma was competitive.
Conclusion:
Mit is a strong inhibitor on DNA polymerase alpha, beta, and gamma. The inhibitory mode was competition with respect to template DNA.
