[Immunohistochemical study on SMDS using anti-actin monoclonal antibody]

For elucidating the relationship between the causes of sudden manhood death syndrome (SMDS) and cardiac sudden death, the cardiac conducting system-(CCS) and myocardium of 23 cases including SMDS group, coronary heart disease group and normal control group were studied using anti-actin monoclonal antibody (HHF35) S-P immunohistochemical method. The results showed that some areas of CCS and myocardium were stained negatively by HHF35 in SMDS group but no infarction area can be found with H.E staining. The negative staining by HHF35 can be also observed in all cases of coronary heart disease group. In control group, the CCS and myocardium stained positively. It indicated that there were pathological changes such as early ischemia or infarction of myocardium in SMDS, which related closely to cardiac sudden death.