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[Genetics and expression stability of exogenous gene construct in transgenic mice]
J P Zhang1, W D Lao, X C Zhang
1Institute of Developmental Biology, Chinese Academy of Sciences, Beijing.
Yi Chuan Xue Bao = Acta Genetica Sinica
|June 22, 1999
Summary
Transgenic mice carrying the HBsAg gene construct showed high integration and expression rates. Stable inheritance was observed, but expression varied in offspring, suggesting complex integration and expression regulation.
Area of Science:
- Biotechnology
- Genetics
- Molecular Biology
Context:
- Development of transgenic animal models is crucial for studying gene function and disease.
- Hepatitis B surface antigen (HBsAg) expression in transgenic models can aid in vaccine development and understanding viral pathogenesis.
Purpose:
- To generate transgenic mice expressing the Hepatitis B surface antigen (HBsAg) using a specific gene construct.
- To assess the integration efficiency, expression levels, and heritability of the introduced gene construct in mice.
Summary:
- Transgenic mice were created by microinjecting an exogene construct (lambda 106) containing the HBsAg gene under the control of the bovine alpha-S1 casein gene promoter.
- PCR-Southern hybridization confirmed a 56% integration rate, and ELISA showed 100% expression of HBsAg in the first-generation transgenic mice.
- Subsequent generations demonstrated stable gene inheritance and expression, although variations in expression levels and integration patterns (potentially multi-site integration) were noted, possibly due to position effects or epigenetic modifications.
Impact:
- This study provides a valuable transgenic mouse model for HBsAg research.
- Findings contribute to understanding the complexities of exogenous gene integration, expression stability, and inheritance in mammals.
- The model has potential applications in hepatitis B research and the development of novel therapeutic strategies.