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Related Experiment Videos

A functional bone morphogenetic protein system in the ovary.

S Shimasaki1, R J Zachow, D Li

  • 1Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA 92093-0633, USA. sshimasaki@ucsd.edu

Proceedings of the National Academy of Sciences of the United States of America
|June 23, 1999
PubMed
Summary

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Bone morphogenetic proteins (BMPs) play a crucial role in rat ovary function. These BMPs differentially regulate follicle-stimulating hormone (FSH) actions, impacting estradiol and progesterone production.

Area of Science:

  • Reproductive biology
  • Endocrinology
  • Molecular biology

Background:

  • Bone morphogenetic proteins (BMPs) are vital for development but their role in reproduction is unclear.
  • A functional BMP system, including ligands and receptors, has been identified in the rat ovary.

Purpose of the Study:

  • To investigate the paracrine function of BMPs in the rat ovary.
  • To determine the effects of BMPs on follicle-stimulating hormone (FSH)-induced granulosa cell differentiation and steroidogenesis.

Main Methods:

  • In situ hybridization histochemistry to localize BMP and receptor mRNA in rat ovaries.
  • Treatment of rat granulosa cells with recombinant BMP-4 and BMP-7 in serum-free medium.
  • Measurement of estradiol and progesterone production in response to FSH and BMPs.

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Main Results:

  • BMP-4 and BMP-7 mRNA were found in theca cells, with receptors in granulosa cells and oocytes.
  • BMPs modulated FSH-stimulated estradiol and progesterone production in a dose- and time-dependent manner.
  • BMPs enhanced FSH's effect on estradiol and attenuated its effect on progesterone, increasing granulosa cell sensitivity to FSH.

Conclusions:

  • BMPs are identified as key regulators of FSH-dependent steroidogenesis in the rat ovary.
  • BMPs differentially regulate estradiol and progesterone production, mirroring normal estrous cycle steroidogenesis.
  • BMPs are hypothesized to function as luteinization inhibitors in developing Graafian follicles.