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Cdc20 associates with the kinase aurora2/Aik

D C Farruggio1, F M Townsley, J V Ruderman

  • 1Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.

Proceedings of the National Academy of Sciences of the United States of America
|June 23, 1999
PubMed

Insights

Cdc20, a key cell cycle regulator, associates with the aurora2 kinase. This interaction suggests aurora2 may regulate Cdc20

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cdc20/fizzy proteins activate the anaphase-promoting complex/cyclosome (APC/C).
  • APC/C drives proteolysis of cell cycle proteins, enabling mitosis exit and chromosome segregation.
  • hCdc20/p55CDC is known to bind kinases, but specific interactions are not fully elucidated.

Purpose of the Study:

  • To investigate the kinase activity associated with Cdc20.
  • To identify specific kinases interacting with human Cdc20 (hCdc20).
  • To explore the functional implications of Cdc20-kinase interactions in cell cycle regulation.

Main Methods:

  • Assay of myelin basic protein kinase activity associated with Cdc20.
  • Identification of interacting kinases using HeLa cell extracts.
  • Analysis of protein localization and cell cycle phase-specific activity.

Main Results:

  • Cdc20-associated kinase activity peaks in M phase (embryonic) or G2 phase (somatic) cells.
  • Human Cdc20 (hCdc20) was found to associate with the aurora2/Aik kinase.
  • Aurora2/Aik is a mitotic spindle-localized kinase involved in chromosome segregation and genomic stability.

Conclusions:

  • Cdc20 interacts with the aurora2 kinase during specific cell cycle phases.
  • This association suggests aurora2 kinase plays a role in regulating Cdc20 function.
  • The findings provide insight into the molecular mechanisms controlling chromosome segregation and mitotic progression.

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