Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure, function and localization of Helicobacter pylori urease.

B E Dunn1, S H Phadnis

  • 1Department of Pathology, Medical College of Wisconsin, Milwaukee, USA.

The Yale Journal of Biology and Medicine
|June 23, 1999
PubMed
Summary

Helicobacter pylori uses "altruistic autolysis" to attach urease to its surface, aiding survival. This communal behavior is crucial for understanding H. pylori pathogenesis and related diseases like gastritis and ulcers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute effects of prostaglandin E1 and E2 on vascular reactivity and blood flow in situ in the chick chorioallantoic membrane.

Prostaglandins, leukotrienes, and essential fatty acids·2012
Same author

Telepathology overview: from concept to implementation.

Human pathology·2002
Same author

Combined robotic and nonrobotic telepathology as an integral service component of a geographically dispersed laboratory network.

Human pathology·2002
Same author

Helicobacter pylori springs another surprise.

Nature structural biology·2001
Same author

Telepathology networking in VISN-12 of the Veterans Health Administration.

Telemedicine journal and e-health : the official journal of the American Telemedicine Association·2000
Same author

Routine surgical telepathology in the Department of Veterans Affairs: experience-related improvements in pathologist performance in 2200 cases.

Telemedicine journal : the official journal of the American Telemedicine Association·2000

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Gastroenterology

Background:

  • Helicobacter pylori is a primary cause of gastritis and associated diseases, including ulcers and gastric cancer.
  • Chronic H. pylori infection is linked to severe gastrointestinal conditions.
  • Urease activity is vital for H. pylori survival and disease development.

Purpose of the Study:

  • To investigate the mechanism of urease association with the H. pylori surface.
  • To understand the role of urease in H. pylori's acid resistance and pathogenesis.
  • To identify novel bacterial survival strategies in H. pylori.

Main Methods:

  • Analysis of urease activity in H. pylori isolates.
  • In vivo and in vitro studies of urease localization.

Related Experiment Videos

  • Characterization of the mechanism of urease surface association.

    • Main Results:

    • Fresh H. pylori isolates exhibit significant urease activity essential for survival.
    • A substantial portion of urease is found on the bacterial surface, crucial for acid resistance.
    • The unique process of "altruistic autolysis" was identified, involving programmed cell lysis and urease re-adsorption.

    Conclusions:

    • "Altruistic autolysis" is a novel mechanism for urease surface association in H. pylori.
    • This communal behavior is essential for H. pylori's survival and pathogenesis.
    • Understanding this mechanism provides critical insights into H. pylori-related diseases.