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Detection of the DeltaF508 mutation in the CFTR gene by means of time-resolved fluorescence methods
S Kirschstein1, S Winter, D Turner
1Institute of Molecular Biotechnology, Department of Single Cell and Single Molecule Techniques, Jena, Germany. omar@imb-jena.de
Abstract:
A rapid recognition in the base sequence of nucleic acids is an important prerequisite toward the diagnosis of genetic diseases and their carrier states. We have developed a hybridisation method in which a fluorescently labeled oligonucleotide is used to detect point mutations in a target by a simple fluorescence lifetime analysis of the emission of the fluorescent label. We applied this method to detect the deltaF508 mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene in a model system and with biologically derived PCR product and discuss the potential generality of this method.