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Related Experiment Videos

Circulating basic fibroblast growth factor declines during Cy/TBI bone marrow transplantation.

P Okunieff1, A J Barrett, S E Phang

  • 1Department of Radiation Oncology, University of Rochester Medical Center, NY, USA.

Bone Marrow Transplantation
|June 26, 1999
PubMed
Summary

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Basic fibroblast growth factor (bFGF) protects tissues from radiation damage. However, this study found that circulating bFGF levels significantly decreased in patients undergoing total body irradiation (TBI) before bone marrow transplantation.

Area of Science:

  • Oncology
  • Radiotherapy
  • Molecular Biology

Background:

  • Basic fibroblast growth factor (bFGF) demonstrates radioprotective properties against radiation-induced apoptosis in various tissues.
  • Chronic bFGF overexpression is linked to potential fibrotic effects.
  • Understanding endogenous bFGF dynamics during radiation therapy is crucial for assessing normal tissue response.

Purpose of the Study:

  • To measure endogenous circulating bFGF levels in patients undergoing conditioning total body irradiation (TBI) prior to allogeneic bone marrow transplantation (BMT).
  • To investigate the changes in plasma bFGF concentrations during and after TBI.
  • To explore the potential correlation between bFGF levels and radiation tolerance.

Main Methods:

  • Blood samples were collected daily from 26 patients with hematologic malignancies undergoing cyclophosphamide/TBI conditioning for allogeneic BMT.

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  • Plasma bFGF levels were quantified using established assays.
  • bFGF concentrations were compared to normal volunteer ranges (0.8-26 pg/ml) and analyzed pre-TBI, during TBI, and post-TBI.

    • Main Results:

    • A significant decrease in median circulating plasma bFGF levels was observed, dropping from 52 pg/ml pre-TBI to 26 pg/ml during TBI and 5 pg/ml post-TBI.
    • bFGF was below detection limits in a substantial proportion of patients: 22% pre-TBI, 30% during TBI, and 45% post-TBI.
    • While some patients exhibited naturally high bFGF levels, 79% had low or undetectable levels in at least one sample, suggesting a general depletion during conditioning.

    Conclusions:

    • Endogenous circulating bFGF levels significantly decrease in patients undergoing TBI for allogeneic BMT.
    • The majority of patients experienced low or undetectable bFGF levels during the conditioning regimen.
    • The precise impact of these endogenous bFGF fluctuations on normal tissue tolerance to irradiation requires further investigation.