Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in sodium-calcium exchange and calcium currents in diabetic rat ventricular myocytes.

S Chattou1, J Diacono, D Feuvray

  • 1Laboratoire de Physiologie Cellulaire, Université Paris XI, Orsay, France.

Acta Physiologica Scandinavica
|June 26, 1999
PubMed
Summary

Diabetic rats show reduced inward sodium-calcium exchange current (INa-Ca) and L-type calcium current (ICa) in heart cells. These changes in ion currents may affect overall heart function in diabetes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diabetes-related metabolic perturbations in cardiac myocyte.

Diabetes & metabolism·2008
Same author

Enhanced activity of the myocardial Na+/H+ exchanger contributes to left ventricular hypertrophy in the Goto-Kakizaki rat model of type 2 diabetes: critical role of Akt.

Diabetologia·2007
Same author

Intracellular sodium increase and susceptibility to ischaemia in hearts from type 2 diabetic db/db mice.

Diabetologia·2006
Same author

Diabetic cardiomyopathy.

Archives des maladies du coeur et des vaisseaux·2004
Same author

Intracellular sodium in the ischemic and reperfused heart and its role in injury.

Magma (New York, N.Y.)·2001
Same author

Changes in intracellular sodium and pH during ischaemia-reperfusion are attenuated by trimetazidine. Comparison between low- and zero-flow ischaemia.

Cardiovascular research·2000

Area of Science:

  • Cardiovascular Physiology
  • Diabetic Cardiomyopathy Research
  • Ion Channel Function

Background:

  • Diabetes mellitus can lead to significant cardiovascular complications.
  • Alterations in ion channel function are implicated in diabetic heart disease.

Purpose of the Study:

  • To investigate changes in inward sodium-calcium exchange current (INa-Ca) and L-type calcium current (ICa) in diabetic rat ventricular myocytes.
  • To understand the functional impact of these ion current alterations in the diabetic heart.

Main Methods:

  • Utilized the nystatin-perforated patch technique for electrophysiological recordings in isolated ventricular myocytes.
  • Induced diabetes in rats using streptozotocin.
  • Measured INa-Ca and ICa using voltage-clamp electrophysiology.

Related Experiment Videos

Main Results:

  • Diabetic myocytes exhibited reduced INa-Ca density compared to normal myocytes.
  • Caffeine-activated INa-Ca was significantly reduced and showed prolonged decay in diabetic cells.
  • L-type calcium current (ICa) density was significantly decreased in diabetic myocytes between -10 and +50 mV.
  • The fast inactivation time constant of ICa was increased in diabetic myocytes, suggesting altered sarcoplasmic reticulum calcium release dynamics.

Conclusions:

  • Diabetes significantly impairs both INa-Ca and ICa in rat ventricular myocytes.
  • Reduced ICa may contribute to lower intracellular calcium levels during systole in diabetic hearts.
  • Impaired ICa and INa-Ca may collectively compromise cardiac contractility and calcium handling in diabetic cardiomyopathy.